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Only perform instrument QC when needed. It is not required each time the sorting nozzle is installed. Even with instrument QC, you can typically run your sample in less than 17 minutes compared to less than 8 minutes without QC.

Automated stream setup and steering into tubes or plates saves valuable minutes. Minimal downtime Instrument QC continuously monitors the system to track performance and predict problems such as laser failures before they occur.

The sorter design facilitates fast troubleshooting, such as easy and quick replacement of the sorting nozzle, with no need to re-align the laser and stream or run QC beads.

You can resume sorting within 3 minutes because the nozzle is separate from the fixed-alignment flow cell, which is where the sample is interrogated.

This affordable solution allows for in-house cell sorting, which can save money compared to outsourcing. Designed to empower scientific discovery Simplified operation does not mean reduced performance.

Detect and sort dim cells and rare cells The patented gel-coupled cuvette with fixed alignment ensures that lasers are precisely focused on the sample stream to generate the greatest signal for optimizing the resolution of dim cells and adjacent populations.

Spatially separated laser beams ensure minimal optical background noise and improved sensitivity from each laser for resolving dim populations.

The sorter can process many cells quickly to improve the isolation of rare cells. Laser and color configurations facilitate multicolor experiments.

Sweet Spot technology maintains sort stream stability so yield and purity are maintained. The values remain independent of experiment settings and are automatically updated and maintained after QC runs.

Management of risk Biological safety in flow cytometry is a growing issue for laboratories concerned about the potential incidental exposure of operators to biological samples.

By controlling and filtering air, BSCs can provide: Protection of personnel from harmful agents inside the cabinet Protection of products to avoid contamination Protection of the environment from contaminants contained within the cabinet A Class II biosafety cabinet provides both personnel and product protection, and addresses regional standards for biosafety cabinet design and performance.

An open-front cabinet with controlled airflow HEPA-filtered, vertical laminar airflow within the work area Exhausted air from the cabinet is filtered by HEPA filters Can be used in BSL 1, 2, and 3 laboratories Comprehensive biosafety solution Our biosafety and sorting solution for the BD FACSMelody Cell Sorter is comprehensive and keeps your cell sorting activities running smoothly and safely, so you can focus on your research.

Reagents Instruments. A Cells were first gated for lineage negative and live cells, followed by doublet cell discrimination not shown.

Untransfected cells were used as a negative control to set the instrument and draw the gates not shown. Post-sort analysis revealed a purity of Peripheral blood mononuclear cells PBMCs were isolated from human whole blood and stained with an antibody cocktail for the identification and sorting of Tregs.

Lymphocytes were first gated based on light scatter, and following doublet discrimination not shown , helper T cells were defined based on CD4 expression.

Post-sort analysis revealed a cell purity of With each fluorochrome excited by a different laser and cross-laser excitation accounted for, spectral overlap between channels was minimal Single-cell deposition.

A Population hierarchy. Area, height and width parameters for side scatter, forward scatter and DAPI were sequentially used to exclude doublets and cell aggregates.

The gating strategy used in this experiment may have underestimated or excluded mitotic figures. B DNA content analysis. C Post-sort validation of single-cell deposition.

Cells within the DAPI singlet gate were sorted into three multiwell plates with